Redox-based probes for protein tyrosine phosphatases

Stephen E. Leonard; Francisco J. Garcia; David S. Goodsell; Kate S. Carroll

doi:10.1002/anie.201007871

Redox-based probes for protein tyrosine phosphatases

Stephen E. Leonard
, Francisco J. Garcia
, David S. Goodsell
, Kate S. Carroll

Research output: Contribution to journal › Article › peer-review

50 Scopus citations

Abstract

Three in one: The design strategy for redox-based probes (RBPs) that detect the reversible oxidation of protein tyrosine phosphatases (PTPs) includes a "warhead" that forms a covalent adduct with the oxidized active site cysteine of PTPs, a synthetic module that directs binding to the PTP active site, and a chemical reporter tag used for the identification, purification, or direct visualization of the probe-labeled proteins (see picture).

Original language	English (US)
Pages (from-to)	4423-4427
Number of pages	5
Journal	Angewandte Chemie - International Edition
Volume	50
Issue number	19
DOIs	https://doi.org/10.1002/anie.201007871
State	Published - May 2 2011
Externally published	Yes

All Science Journal Classification (ASJC) codes

Catalysis
General Chemistry

Keywords

chemoselectivity
cysteine oxidation
protein modifications
redox chemistry
tyrosine phosphatase

Access to Document

10.1002/anie.201007871

Cite this

@article{bc09c8c8f79c47a99dd957b359b6bcf0,

title = "Redox-based probes for protein tyrosine phosphatases",

abstract = "Three in one: The design strategy for redox-based probes (RBPs) that detect the reversible oxidation of protein tyrosine phosphatases (PTPs) includes a {"}warhead{"} that forms a covalent adduct with the oxidized active site cysteine of PTPs, a synthetic module that directs binding to the PTP active site, and a chemical reporter tag used for the identification, purification, or direct visualization of the probe-labeled proteins (see picture).",

keywords = "chemoselectivity, cysteine oxidation, protein modifications, redox chemistry, tyrosine phosphatase",

author = "Leonard, \{Stephen E.\} and Garcia, \{Francisco J.\} and Goodsell, \{David S.\} and Carroll, \{Kate S.\}",

year = "2011",

month = may,

day = "2",

doi = "10.1002/anie.201007871",

language = "English (US)",

volume = "50",

pages = "4423--4427",

journal = "Angewandte Chemie - International Edition",

issn = "1433-7851",

publisher = "John Wiley and Sons Ltd",

number = "19",

}

TY - JOUR

T1 - Redox-based probes for protein tyrosine phosphatases

AU - Leonard, Stephen E.

AU - Garcia, Francisco J.

AU - Goodsell, David S.

AU - Carroll, Kate S.

PY - 2011/5/2

Y1 - 2011/5/2

N2 - Three in one: The design strategy for redox-based probes (RBPs) that detect the reversible oxidation of protein tyrosine phosphatases (PTPs) includes a "warhead" that forms a covalent adduct with the oxidized active site cysteine of PTPs, a synthetic module that directs binding to the PTP active site, and a chemical reporter tag used for the identification, purification, or direct visualization of the probe-labeled proteins (see picture).

AB - Three in one: The design strategy for redox-based probes (RBPs) that detect the reversible oxidation of protein tyrosine phosphatases (PTPs) includes a "warhead" that forms a covalent adduct with the oxidized active site cysteine of PTPs, a synthetic module that directs binding to the PTP active site, and a chemical reporter tag used for the identification, purification, or direct visualization of the probe-labeled proteins (see picture).

KW - chemoselectivity

KW - cysteine oxidation

KW - protein modifications

KW - redox chemistry

KW - tyrosine phosphatase

UR - https://www.scopus.com/pages/publications/79955493857

UR - https://www.scopus.com/pages/publications/79955493857#tab=citedBy

U2 - 10.1002/anie.201007871

DO - 10.1002/anie.201007871

M3 - Article

C2 - 21504031

AN - SCOPUS:79955493857

SN - 1433-7851

VL - 50

SP - 4423

EP - 4427

JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

IS - 19

ER -

Redox-based probes for protein tyrosine phosphatases

Abstract

All Science Journal Classification (ASJC) codes

Keywords

Access to Document

Other files and links

Fingerprint

Cite this