Isolating the cDNA fragment inhibiting nontargeted mutagenesis in vero cell by antisense technology

Wenwei Hu; Yingnian Yu; Xingruo Chen; Tao Song; Haiyang Xie

doi:10.1007/BF02885542

Isolating the cDNA fragment inhibiting nontargeted mutagenesis in vero cell by antisense technology

Wenwei Hu
, Yingnian Yu
, Xingruo Chen
, Tao Song
, Haiyang Xie

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Among the expressed sequence tags (ESTs) which were isolated from genetically instable monkey kidney vero cells induced by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), a fragment 9 was isolated. When the expression of fragment 9 was blocked through transcripting antisense RNA, MNNG-induced nontargeted mutation frequency enhanced significantly compared with control (P < 0.05) . The result showed that the relevant gene of fragment 9 might participate in maintaining cellular genetic stability and be involved in inhibition of nontargeted mutagenesis.

Original language	English (US)
Pages (from-to)	533-537
Number of pages	5
Journal	Chinese Science Bulletin
Volume	44
Issue number	6
DOIs	https://doi.org/10.1007/BF02885542
State	Published - Mar 1999
Externally published	Yes

All Science Journal Classification (ASJC) codes

General

Keywords

Antisense RNA
Genetic instability
Mutation
Nontargeted

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10.1007/BF02885542

Cite this

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title = "Isolating the cDNA fragment inhibiting nontargeted mutagenesis in vero cell by antisense technology",

abstract = "Among the expressed sequence tags (ESTs) which were isolated from genetically instable monkey kidney vero cells induced by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), a fragment 9 was isolated. When the expression of fragment 9 was blocked through transcripting antisense RNA, MNNG-induced nontargeted mutation frequency enhanced significantly compared with control (P < 0.05) . The result showed that the relevant gene of fragment 9 might participate in maintaining cellular genetic stability and be involved in inhibition of nontargeted mutagenesis.",

keywords = "Antisense RNA, Genetic instability, Mutation, Nontargeted",

author = "Wenwei Hu and Yingnian Yu and Xingruo Chen and Tao Song and Haiyang Xie",

note = "Funding Information: Acknowledgment This work was supported by the National Natural Science Foundation of China (Grant No. Natural Science Foundation of Zhejiang Province (Grant No. 394191).",

year = "1999",

month = mar,

doi = "10.1007/BF02885542",

language = "English (US)",

volume = "44",

pages = "533--537",

journal = "Chinese Science Bulletin",

issn = "1001-6538",

publisher = "Science Press",

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}

TY - JOUR

T1 - Isolating the cDNA fragment inhibiting nontargeted mutagenesis in vero cell by antisense technology

AU - Hu, Wenwei

AU - Yu, Yingnian

AU - Chen, Xingruo

AU - Song, Tao

AU - Xie, Haiyang

N1 - Funding Information: Acknowledgment This work was supported by the National Natural Science Foundation of China (Grant No. Natural Science Foundation of Zhejiang Province (Grant No. 394191).

PY - 1999/3

Y1 - 1999/3

N2 - Among the expressed sequence tags (ESTs) which were isolated from genetically instable monkey kidney vero cells induced by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), a fragment 9 was isolated. When the expression of fragment 9 was blocked through transcripting antisense RNA, MNNG-induced nontargeted mutation frequency enhanced significantly compared with control (P < 0.05) . The result showed that the relevant gene of fragment 9 might participate in maintaining cellular genetic stability and be involved in inhibition of nontargeted mutagenesis.

AB - Among the expressed sequence tags (ESTs) which were isolated from genetically instable monkey kidney vero cells induced by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), a fragment 9 was isolated. When the expression of fragment 9 was blocked through transcripting antisense RNA, MNNG-induced nontargeted mutation frequency enhanced significantly compared with control (P < 0.05) . The result showed that the relevant gene of fragment 9 might participate in maintaining cellular genetic stability and be involved in inhibition of nontargeted mutagenesis.

KW - Antisense RNA

KW - Genetic instability

KW - Mutation

KW - Nontargeted

UR - https://www.scopus.com/pages/publications/1542669623

UR - https://www.scopus.com/pages/publications/1542669623#tab=citedBy

U2 - 10.1007/BF02885542

DO - 10.1007/BF02885542

M3 - Article

AN - SCOPUS:1542669623

SN - 1001-6538

VL - 44

SP - 533

EP - 537

JO - Chinese Science Bulletin

JF - Chinese Science Bulletin

IS - 6

ER -

Isolating the cDNA fragment inhibiting nontargeted mutagenesis in vero cell by antisense technology

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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